Composite

Part:BBa_K5437019:Design

Designed by: Yu-Li He   Group: iGEM24_Formosa   (2024-09-28)


Overexpression proteins for originally rate limiting steps of MVA, MEP, GPP, and L-borneol Biosynthe


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5412
    Illegal SpeI site found at 83
    Illegal PstI site found at 5234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5412
    Illegal SpeI site found at 83
    Illegal PstI site found at 5234
    Illegal NotI site found at 5267
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5412
    Illegal BglII site found at 9083
    Illegal BglII site found at 10861
    Illegal BglII site found at 11495
    Illegal BamHI site found at 4328
    Illegal XhoI site found at 11742
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5412
    Illegal SpeI site found at 83
    Illegal PstI site found at 5234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5412
    Illegal SpeI site found at 83
    Illegal PstI site found at 5234
    Illegal NgoMIV site found at 1913
    Illegal NgoMIV site found at 10959
    Illegal AgeI site found at 10164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6846
    Illegal BsaI.rc site found at 9759


Design Notes

T7 promoters and RBS sites strategically placed to promote maximum efficiency of transcription.


Source

Constructed basic parts in multiple cutting sites of plasmid backbone and attached T7 promoter

References